Which one of the following occurs during the conversion of pyruvate to glucose during gluconeogenesis?
选项：

A:Biotin is required as a cofactor.
B: The carbon of CO2, added in one reaction, appears in the final product.
C:Energy is utilized only in the form of GTP
D: All of the reactions occur in the cytosol.
E: All of the reactions occur in the mitochondrion.